comparison of biocompatibility of various membranes with fibroblasts

نویسندگان

طاهره فروتن

t. foroutan faculty of biological sciences, kharazmi university, tehran- iran.گروه زیست شناسی جانوری، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران محمود قاسمی

m. ghasemi dept. of periodontology, dental school, islamic azad university, tehran- iran.گروه پریودنتولوژی، دانشکده دندانپزشکی، دانشگاه ازاد اسلامی، واحد تهران سولماز فرهنگ

s. farhang dental school, islamic azad university, tehran- iran.دانشکده دندانپزشکی، دانشگاه آزاد اسلامی، تهران- ایران.

چکیده

objective: different techniques have been suggested for the repair of bone defects at the injured sites. use of biomembranes, or application of plasma rich in growth factor (prgf) at the site of proliferation of osteoblasts are among the suggested techniques. the current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts (hplf) cultured on hypro-sorb f, pericardium and tutodent resorbable membranes coated with prgf.   methods: this experimental study was conducted on four resorbable membranes namely hypro-sorb f, pericardium, tutodent and vicryl. fibroblast cells isolated from the periodontal ligament of premolar tooth were passaged three times and 105 cells/cm2 were cultured on membranes coated with prgf. after 72 hours, the cells were evaluated in terms of biocompatibility and alkaline phosphatase (alp) activity. statistical analysis was carried out using one-way anova.   results: prgf increased cell adhesion and tutodent membrane coated with prgf showed the highest cell adhesion compared with hypro-sorb f and pericardium membranes (p=0.005).   conclusion: prgf increases cell viability and alp activity of cells on biomembranes. prgf treatment increases the adhesion of fibroblast cells to these membranes.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Comparison of Biocompatibility of Various Membranes with Fibroblasts

  Objective: Different techniques have been suggested for the repair of bone defects at the injured sites. Use of biomembranes, or application of plasma rich in growth factor (PRGF) at the site of proliferation of osteoblasts are among the suggested techniques. The current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts (hPLF) cultured on Hypro-Sorb F, Peri...

متن کامل

Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells.

The aim of the present study was to evaluate the biocompatibility of differently cross-linked collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Four collagen membranes [BioGide (BG), BioMend (BM), Ossix (OS) and TutoDent (TD)] were tested. Cells plated on culture dishes (CD) served as positive controls. Six specimens of each membrane were incubated with (1...

متن کامل

a comparison of teachers and supervisors, with respect to teacher efficacy and reflection

supervisors play an undeniable role in training teachers, before starting their professional experience by preparing them, at the initial years of their teaching by checking their work within the proper framework, and later on during their teaching by assessing their progress. but surprisingly, exploring their attributes, professional demands, and qualifications has remained a neglected theme i...

15 صفحه اول

comparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.

heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...

15 صفحه اول

Biocompatibility of hemodialysis membranes.

Exposure of blood to hemodialysis membranes results in numerous interactions between the blood elements and the membrane. Transformation and adsorption of plasma proteins (such as complement) and activation of blood cells (such as neutrophils and monocytes) have been studied most extensively by nephrologists in recent years. There is no consensus on the definition of biocompatibility for dialyz...

متن کامل

a comparison of linguistic and pragmatic knowledge: a case of iranian learners of english

در این تحقیق دانش زبانشناسی و کاربردشناسی زبان آموزان ایرانی در سطح بالای متوسط مقایسه شد. 50 دانش آموز با سابقه آموزشی مشابه از شش آموزشگاه زبان مختلف در دو آزمون دانش زبانشناسی و آزمون دانش گفتار شناسی زبان انگلیسی شرکت کردند که سوالات هر دو تست توسط محقق تهیه شده بود. همچنین در این تحقیق کارایی کتابهای آموزشی زبان در فراهم آوردن درون داد کافی برای زبان آموزان ایرانی به عنوان هدف جانبی تحقیق ...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of dental school, shahid beheshti university of medical sciences

جلد ۳۲، شماره ۳، صفحات ۱۴۵-۱۵۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023